Nucleic Acids Research, 1990, Vol. 18, No. 2 400
© 1990
Corrigenda |
Direct sequencing from low-melt agarose with Sequenase®
Two important points were omitted from the methods details of this article. The third sentence of the second paragraph should read:
10 µl of DNA were annealed to 5 µl of sequencing primer (10 ng/µl) and 3 µl of sequencing buffer (5x) by heating to 95°C (or placing in boiling water) for 10 min and cooling quickly to 37°C.
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