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Nucleic Acids Research, 1990, Vol. 18, No. 20 5955-5960
© 1990

CHEMISTRY

Synthesis and NMR study of ribooligonucleotides forming a hammerhead-type RNA enzyme system

Osamu Odai, Hirofumi Kodama, Hidekazu Hiroaki, Takeshi Sakata, Toshiki Tanaka1 and Seiichi Uesugi*

Faculty of Pharmaceutical Sciences, Osaka University 1-6 Yamadaoka, Suita, Osaka 565, Japan 1Protein Engineering Research Institute 6-23 Furuedai, Suita, Osaka 565, Japan

*To whom correspondence should be addressed

Received August 13, 1990. Revised September 11, 1990. We designed a hammerhead-type ribozyme system which consists of three RNA fragments and synthesized the component andrelated ribooligonucleotides by the solid-phase phosphoramidite method using o-nitrobenzyl groups for 2'-hydroxyl protection. Improved conditions for photolytic removal of the o-nitrobenzyl groups are described. Imino proton NMR spectra of the ribozyme complex were measured. The resonances for the hydrogen-bonded imino protons were assigned by comparison with the spectra of model complexes which contain the base sequences of one or two of the stem regions. The results suggest that the complex indeed forms a hammerhead structure and the loop regions take an ordered conformation in the absence of magnesium ions.


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