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Nucleic Acids Research, 1990, Vol. 18, No. 20 6075-6081
© 1990

MOLECULAR BIOLOGY

Synthesis of a gene for human serum albumin and its expression in Saccharomyces cerevisiae

Miklós Kálmán1, Imre Cserpän1, György Bajszár2, Albert Dobi2, Éva Horváth2, Cecilia Pázmán2 and András Simoncsits1,3,*

1Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences H-6701 Szeged, PO Box 521, Hungary 2Laboratory of Molecular Biology, Vepex-Biotechnika Ltd. H-6701 Szeged, PO Box 521, Hungary 3Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University S-106 91 Stockholm, Sweden

*To whom correspondence should be addressed

Received June 11, 1990. Revised September 25, 1990. Accepted September 25, 1990.

A 1761 base pairs long artificial gene coding for human serum albumin (HSA) has been prepared by a newly developed synthetic approach, resulting in the largest synthetic gene so far described. Oligonucleotides corresponding to only one strand of the HSA gene were prepared by chemical synthesis, while the complementary strand was obtained by a combination of enzymatic and cloning steps. 24 synthetic, 69–85 nucleotldes long oligonucleotides covering the major part of the HSA gene (41–1761 nucleotides) were used as building blocks. Generally, four groups of 6–6 such oligonucleotides were successively cloned in pUC19 Escherichia coli vector to obtain about quarters of the gene aslarge fragments. Joining of these four fragments resulted in a cloned DNA coding for the 13–585 amino acid region of HSA, which was further supplemented with a double-stranded linker sequence coding for the amino terminal 12 amino acids. The completed structural gene composed of frequently used codons in the highly expressed yeast genes was then supplied with yeast regulatory sequences and the HSA expression cassette so obtained was inserted into an Escherichla coll – Saccharomyces cerevisiae shuttle vector. This vector was shown to direct the expression in Saccharomyces cerevisiae of correctly processed, mature HSA which was recognized by antiserum to HSA, and possessed the correct N-terminalamino acid sequence.


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