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Nucleic Acids Research, 1990, Vol. 18, No. 21 6211-6214
© 1990

GENOME STRUCTURE AND MAPPING

Size and physical map of the Campylobacter jejuni chromosome

Piet J.M. Nuijten, Corien Bartels, Nancy M.C. Bleumink-Pluym, Wim Gaastra and Bernard A.M. van der Zeijst*

Department of Bacteriology of the Institute of Infectious Diseases and Immunology, School of Veterinary Medicine, University of Utrecht PO Box 80.165, 3508 TD Utrecht, The Netherlands

*To whom correspondence should be addressed

Received August 28, 1990. Accepted October 3, 1990.

The chromosome of Campylobacter Jejuni is circular and approximately 1700 kb in circumference. The size of the genome was determined by field inversion gel electrophoresis of restriction endonuclease fragments using lambda DNA concatamers and yeast chromosomes to calibrate the size of the fragments. In view of the low (32–35%) G + C content of the campylobacter genome, enzymes that recognizes GC-rich sequences were used. Of the enzymes tested BssHII (G/CGCGC), Nc/Il(Formula) and Sali (G/TCGAC) appeared to be usable. Hybridization of labeled fragments with two or more fragments from digests with a different restriction enzyme gave the information to order the fragments on the C.jejuni chromosome. The localization on the genome of the flagellin and ribosomal gene clusters was determined.


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