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Nucleic Acids Research, 1990, Vol. 18, No. 21 6277-6282
© 1990

MOLECULAR BIOLOGY

The {alpha}-foetoprotein proximal enhancer: localization, cell specificity and modulation by dexamethasone

Corinne Houart, Josiane Szpirer and Claude Szpirer*

Département de Biologie Moléculaire, Université Libre de Bruxelles Rue des Chevaux, 67, B-1640 Rhode-St-Genése, Belgium

*To whom correspondence should be addressed

Received July 26, 1990. Revised October 9, 1990. Accepted October 9, 1990.

The enhancer element present in the 5'proximal region flanking the mouse {alpha}-foetoprotein (AFP) gene, active in AFP-producing hepatoma cells and inactive in non-producing hepatoma cells, was localized between positions –203 and –79. This enhancer segment contains a sequence resembling the steroid hormone response element. We demonstrated that this sequence is dispensable for the enhancer activity but mediates dose-dependent effects of dexamethasone on the enhancer activity: dexamethasone decreases the proximal enhancer activity at low concentrations but this inhibitory effect vanishes at high concentrations. Our results indicate that several transcrlptlonal factors, one of which is absent in AFP-non-producing hepatoma cells, control the AFP proximal enhancer activity.


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