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Nucleic Acids Research, 1990, Vol. 18, No. 21 6299-6304
© 1990

MOLECULAR BIOLOGY

Field inversion gel electrophoresis with different pulse time ramps

Christoph Heller* and Fritz M. Pohl

Fakultät für Biologie, Universität Konstanz Postfach 5560, D-7750 Konstanz, FRG

*To whom correspondence should be addressed at Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

Received July 17, 1999. Revised September 3, 1990. Accepted September 3, 1990.

The influence of different pulse time ramps on the separation of yeast chromosomes with field inversion gel electrophoresis (FIGE) was investigated by the means of two dimensional gel electrophoresis. The problem of band inversion, which makes it difficult to distinguish DNA molecules of different size, has been solved by using double randomized pulse times. A major disadvantage of the field inversion technique is thereby overcome, making this system comparable to other pulsed field techniques.


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