Nucleic Acids Research, 1990, Vol. 18, No. 21 6385-6391
© 1990
MOLECULAR BIOLOGY |
Genetic exchange between endogenous and exogenous LINE-1 repetitive elements in mouse cells
Canadian Red Cross Society, Research and Development, Montreal Centre 3131 Sherbrooke St. E, Montreal, Quebec H1W 1B2 1Department of Microbiology and Immunology, Université de Montreal Montreal, Canada
*To whom correspondence should be addressed
Received June 13, 1990. Revised September 11, 1990. Accepted September 11, 1990.
The repetitive LINE (L1) elements of the mouse, which are present at about 105 copies per genome and share over 80% of sequence homology, were examined for their ability to undergo genetic exchange with exogenous L1 sequences. The exogenous L1 sequences, carried by a shuttle vector, consisted of an internal fragment from L1 Md-A2, a previously described member of the L1 family of the mouse. Using an assay that does not require the reconstitution of a selectable marker we found that this vector, in either circular or linear form, acquired DNA sequences from endogenous L1 elements at a frequency of 10–3 to 10–4 per rescued vector. Physical analysis of the acquired L1 sequences revealed that distinct endogenous L1 elements acted as donors and that different subfamilies participated. These results demonstrate that L1 elements are readily capable of genetic exchange. Apart from gene conversion events, the acquisition of L1 sequences outside the region of homology suggested that a second mechanism was also involved in the genetic exchange. A model which accounts for this mechanism is presented and its potential implication on the rearrangement of L1 elements is discussed.
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