Skip Navigation

This Article
Right arrow Print PDF (5411K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (24)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mörl, M.
Right arrow Articles by Schmelzer, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mörl, M.
Right arrow Articles by Schmelzer, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 22 6545-6551
© 1990

MOLECULAR BIOLOGY

Group II intron RNA-catalyzed recombination of RNA in vitro

M. Mörl and C. Schmelzer*

Institut für Genetik und Mikrobiologie der Universität München Maria-Ward-Straße 1a, D-8000 Munich 19, FRG

*To whom correspondence should be addressed

Received August 13, 1990. Revised September 7, 1990. Accepted September 7, 1990.

We report the first evidence for a novel reaction mediated by the self-splicing yeast mitochondrial group II intron bl1; the site-specific recombination of RNA molecules in vitro. Upon incubation of the intron lariat with two different RNAs, each harbouring a short sequence complementary to exon binding site 1 (EBS1) of the intron, novel recomblned RNAs are formed. As a result of this intrort-mediated shuffling of gene segments, the 5' part of RNA1 is ligated to the 3' part of RNA2 and, reciprocally, the 5' part of RNA2 to the 3' part of RNA1. Sequence analysis of the recombinant junction shows that the site of recombination is precisely located 3' to intron binding site 1 (IBS1). The hypothesized mechanism of recombination involves exchange of RNA 5' parts after the first step of a reverse splicing reaction. The possible role of this mechanism in vivo and during prebiotic evolution is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
W. E. Draper, E. J. Hayden, and N. Lehman
Mechanisms of covalent self-assembly of the Azoarcus ribozyme from four fragment oligonucleotides
Nucleic Acids Res., February 2, 2008; 36(2): 520 - 531.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
E. J. HAYDEN, C. A. RILEY, A. S. BURTON, and N. LEHMAN
RNA-directed construction of structurally complex and active ligase ribozymes through recombination
RNA, November 1, 2005; 11(11): 1678 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. Mueller, M Hetzer, and R. Schweyen
Group II intron RNA catalysis of progressive nucleotide insertion: a model for RNA editing
Science, August 20, 1993; 261(5124): 1035 - 1038.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-J. Kim and C. Kao
Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: Implications for RNA-RNA recombination
PNAS, April 24, 2001; 98(9): 4972 - 4977.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.