Nucleic Acids Research, 1990, Vol. 18, No. 23 6799-6806
© 1990
Articles |
Characterization of a functional promoter for the human retinoic acid receptor-alpha (hRAR-
)
Laboratoire de Gënëtique Molëculaire des Eucaryotes du CNRS, Unitë de Biologie Molëculaire et de Gënie Gënëtique de I'INSERM, Institut de Chimie Biologique, Facultë de Mëdecine 11, rue Humann, 67085 Strasbourg Cëdex, France
* To whom correspondence should be addressed
Received September 27, 1990. Accepted October 30, 1990.
The three retinoic acid receptors RAR-
, ß and 
are thought to mediate the effects of RA in vivo. We have determined here the exon organisation in the 5' region of the human RAR- (hRAR-) gene, and have identified its promoter. This promoter drives the expression of promoterless ß-globin or CAT reporter genes when transfected into HeLa, Cos-1 or mouse embryonal carcinoma (EC) P19.6 cells in culture. There are no TATA or CCAAT-box elements In this promoter, which appears to belong to the class of promoters made up of an initiator element preceded by several putative binding sites for the transcription factor Sp1. In addition, the hRAR- promoter region contains a number of sequences that are similar to known enhancer elements. Notably, the hRAR- promoter contains a sequence identical to a binding site for the Krox-20 transcription factors, a zinc finger-containing protein which is thought to play a role in the early development of the mouse central nervous system.
+Present addresse: Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, SW3 6LY, UK
Present addresse: Cancer Research Laboratory, Queens University, Kingston, Ontario K7L 3N6, Canada
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