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Nucleic Acids Research, 1990, Vol. 18, No. 23 6903-6907
© 1990

Articles

Down modulation of HIV-1 gene expression using a procaryotic RNA-binding protein

Ben Berkhout* and Kuan-Teh Jeang

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health Bethesda, MD 20892, USA

* To whom correspondence should be addressed

Received September 6, 1990. Revised October 22, 1990. Accepted October 22, 1990.

The coat protein of the single stranded RNA bacteiiophages acts as a translational repressor by binding with high affinity to a target RNA that encompasses the ribosomal binding site of the replicase gene. We have expressed this procaryotic RNA-binding protein In mammalian cells. Using the coat protein binding site attached to the HIV-1 5' leader RNA, we tested for the biological effect of co-expressed bacteriophage protein. We found that HIV-1 LTR-dlrected expression within this context was inhibited in trans by the coat protein. This example suggests the feasibility of using procaryotic RNA-binding proteins as genetic modulators in eucaryotic cells.


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