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Nucleic Acids Research, 1990, Vol. 18, No. 23 6943-6951
© 1990

Articles

BAP, a rat liver protein that activates transcription through a promoter element with similarity to the USF/MLTF binding site

Wilfried Kugler+, Michael Kaling§, Ulrike Wagner and Gerhart U. Ryffel1,*

Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie Postfach 36 40, D-7500 1Karlsruhe and Institut für Zellbiologie (Tumorforschung), Universitätsklinikum Essen Hufelandstrasse 55, D-4300 Essen 1, FRG

* To whom correspondence should be addressed

Received August 23, 1990. Revised October 23, 1990. Accepted October 23, 1990.

The vitellogenin genes of Xenopus are llver-specifically expressed. An in vitro transcription system derived from rat liver nuclei allowed us to define the cis-element BABS (B-activator binding site) in the promoter of the B1 vitellogenin gene. An oligonucleotide encompassing the region from – 53 to –44 linked to a TATA box is sufficient for a tenfold increase of the transcriptional activity. Gel retardation assays with nuclear rat liver proteins reveal two DNA-protein complexes: Complex 1 can be competed by the USF/MLTF binding site of the adeno major late promoter whereas complex 2 is a distinct protein we refer to as BAP (B-actlvator protein). In vitro transcription experiments in the presence of USF/MLTF binding site as competitor show that BAP is an efficient transcription factor. Based on UV crosslinking we estimate that BAP has a molecular weight of 58 kd. Phosphatase treatment reveals that DNA binding of BAP requires phosphorylation. BABS is also present in the hepatitis B virus enhancer suggesting that it might play a role in the tumorigenic potential of the virus.

+ Sektion Molekularbiologie, Abt. Kinderheilkunde II, Helmholtzstr. 10, D-7900 Ulm

§ Pharmakologisches Institut, Universität Heidelberg, Im Neuenheimer Feld 366, D-6900 Heidelberg, FRG


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