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Nucleic Acids Research, 1990, Vol. 18, No. 23 7093-7097
© 1990

Articles

Developmental regulation of the human zeta globin gene in transgenic mice

E.A. Spangler, K.A. Andrews and E.M. Rubin*

Divisions of Cell and Molecular Biology and Research Medicine, University of California, Lawrence Berkeley Laboratory Berkeley, CA 94720, USA

* To whom correspondence should be addressed

Received June 22, 1990. Revised November 9, 1990. Accepted November 9, 1990.

We have characterized the expression of the human zeta ({zeta}) gene, which encodes an embryonic {zeta}-like giobin, in transgenlc mice. We find that a 777 base pair fragment spanning erythroid specific hypersensitive site II (HSII) from the distal 5 region of the human ß globin gene ciuster potentiates expression of the {zeta} giobin gene. In the absence of the HSII fragment, no expression is observed. Expression of the human {zeta} gene in mice parallels expression of a murine embryonic {alpha}-like giobin gene (x). Thus, expression of the human {zeta} gene in mice requires linkage to an erythroid-specific enhancer sequence, but the presence of the enhancer does not affect the deveiopmental regulation of the transgene. Our results indicate that the factors involved In switching from embryonic to adult {alpha} globin gene expression during development are evolutionarily conserved, and suggest that the transgenic mouse Is an in vivo system In which the requirements for the developmental switch in {alpha} giobin gene expression can be analyzed in detail.


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