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Nucleic Acids Research, 1990, Vol. 18, No. 23 7127-7131
© 1990

Articles

Synthesis and enzymatic polymerisation of 5-amino-1-(2'-deoxy-ß-D-ribofuranosyl)imidazole-4-carboxamide-5'-triphosphate

Sylvie Pochet and Richard D'Ari1

Institut Pasteur, Unité de Chimie Organique URA-CNRS 487, 28 rue du Docteur Roux, 75757 Paris Cedex 15, France 1Institut Jacques Monod, CNRS Université Paris 7, 2 place Jussieu, 75251 Paris Cedex 05, France

Received September 20, 1990. Revised November 2, 1990. Accepted November 2, 1990.

The chemical synthesis of 5-amino-1-(2'-deoxy-ß-D-ribofuranosyl)imidazole-4-carboxamide, referred to as dZ, and of its 5'-triphosphate derivative (dZTP), from 2'-deoxyinosine is described. The polymerisation of dZTP using terminal deoxynucleotidyltransferase to give a homopolymer is also presented.


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