Nucleic Acids Research, 1990, Vol. 18, No. 24 7213-7218
© 1990
Articles |
Fingerprinting genomes using PCR with arbitrary primers*
California Institute of Biological Research 11099 North Torrey Pines Road, La Jolla, CA 92037, USA
Received July 27, 1990. Revised August 30, 1990. Accepted August 30, 1990.
Simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (PCR). No prior sequence information is required. The method, arbitrarily primed PCR (AP-PCR), involves two cycles of low stringency amplification followed by PCR at higher stringency. We show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. The generality of the method Is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa (rice).
*Publication of this paper was delayed by the authors to allow simultaneous publication with a paper submitted later by another group. Nucleic Acids Research regrets that due to administrative errors the other paper, by Williams et al., was published on pages 6531–6535 of issue 22. Both sets of authors agree that the two papers should be considered as published simultaneously and should be referred to together.
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