Nucleic Acids Research, 1990, Vol. 18, No. 24 7267-7277
© 1990
Articles |
Localization and structural analysis of the ribosomal RNA operons of Rhodobacter sphaeroides
Department of Microbiology, University of Texas Health Science Center at Houston Houston, TX 77225, USA
*To whom correspondence should be addressed
Received September 28, 1990. Revised November 7, 1990. Accepted November 7, 1990.
We have identified, cloned and sequenced the three ribosomal RNA (rRNA) operons (rrn) present in the facultative photoheterotroph Rhodobacter sphaeroides. DNA sequence analysis has identified the 16S, 23S, and 5S rRNAs, two tRNAs (ile and ala) in the spacer region between the 16S and 23S rRNAs, and an f-met tRNA immediately following the 5S rRNA gene of all three operons. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. These findings are discussed in relation to the origins of diploidy.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Eraso, J. H. Roh, X. Zeng, S. J. Callister, M. S. Lipton, and S. Kaplan Role of the Global Transcriptional Regulator PrrA in Rhodobacter sphaeroides 2.4.1: Combined Transcriptome and Proteome Analysis J. Bacteriol., July 15, 2008; 190(14): 4831 - 4848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-J. Kim, J.-S. Kim, I.-H. Lee, H. J. Rhee, and J. K. Lee Superoxide Generation by Chlorophyllide a Reductase of Rhodobacter sphaeroides J. Biol. Chem., February 15, 2008; 283(7): 3718 - 3730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Poggio, C. Abreu-Goodger, S. Fabela, A. Osorio, G. Dreyfus, P. Vinuesa, and L. Camarena A Complete Set of Flagellar Genes Acquired by Horizontal Transfer Coexists with the Endogenous Flagellar System in Rhodobacter sphaeroides J. Bacteriol., April 15, 2007; 189(8): 3208 - 3216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Popp, M. Schlomann, and M. Mau Bacterial diversity in the active stage of a bioremediation system for mineral oil hydrocarbon-contaminated soils. Microbiology, November 1, 2006; 152(Pt 11): 3291 - 3304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Tavano, A. M. Podevels, and T. J. Donohue Identification of Genes Required for Recycling Reducing Power during Photosynthetic Growth J. Bacteriol., August 1, 2005; 187(15): 5249 - 5258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Acinas, L. A. Marcelino, V. Klepac-Ceraj, and M. F. Polz Divergence and Redundancy of 16S rRNA Sequences in Genomes with Multiple rrn Operons J. Bacteriol., May 1, 2004; 186(9): 2629 - 2635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Choudhary, Y.-X. Fu, C. Mackenzie, and S. Kaplan DNA Sequence Duplication in Rhodobacter sphaeroides 2.4.1: Evidence of an Ancient Partnership between Chromosomes I and II J. Bacteriol., April 1, 2004; 186(7): 2019 - 2027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Battermann, C. Disse-Kromker, and B. Dreiseikelmann A functional plasmid-borne rrn operon in soil isolates belonging to the genus Paracoccus Microbiology, December 1, 2003; 149(12): 3587 - 3593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Broughton Roses by Other Names: Taxonomy of the Rhizobiaceae J. Bacteriol., May 15, 2003; 185(10): 2975 - 2979. [Full Text] [PDF]
|
|
|
|
|
|
P. van Berkum, Z. Terefework, L. Paulin, S. Suomalainen, K. Lindstrom, and B. D. Eardly Discordant Phylogenies within the rrn Loci of Rhizobia J. Bacteriol., May 15, 2003; 185(10): 2988 - 2998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. J. Gordon, T. Sjogren, M. Lofqvist, C. D. Richter, J. W. A. Allen, C. W. Higham, J. Hajdu, V. Fulop, and S. J. Ferguson Structure and Kinetic Properties of Paracoccus pantotrophus Cytochrome cd1 Nitrite Reductase with the d1 Heme Active Site Ligand Tyrosine 25 Replaced by Serine J. Biol. Chem., March 28, 2003; 278(14): 11773 - 11781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gomelsky, J. Sram, O. V. Moskvin, I. M. Horne, H. N. Dodd, J. M. Pemberton, A. G. McEwan, S. Kaplan, and M. Gomelsky Identification and in vivo characterization of PpaA, a regulator of photosystem formation in Rhodobacter sphaeroides Microbiology, February 1, 2003; 149(2): 377 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rios-Velazquez, R. Coller, and T. J. Donohue Features of Rhodobacter sphaeroides CcmFH J. Bacteriol., January 15, 2003; 185(2): 422 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Klein, R. Samorski, G. Klug, and E. Evguenieva-Hackenberg Atypical Processing in Domain III of 23S rRNA of Rhizobium leguminosarum ATCC 10004T at a Position Homologous to an rRNA Fragmentation Site in Protozoa J. Bacteriol., June 15, 2002; 184(12): 3176 - 3185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Pronk and K. E. Sanderson Intervening Sequences in rrl Genes and Fragmentation of 23S rRNA in Genera of the Family Enterobacteriaceae J. Bacteriol., October 1, 2001; 183(19): 5782 - 5787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Cox, C. Patterson, and T. J. Donohue Roles for the Rhodobacter sphaeroides CcmA and CcmG Proteins J. Bacteriol., August 1, 2001; 183(15): 4643 - 4647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Boyer, V. R. Flechtner, and J. R. Johansen Is the 16S-23S rRNA Internal Transcribed Spacer Region a Good Tool for Use in Molecular Systematics and Population Genetics? A Case Study in Cyanobacteria Mol. Biol. Evol., June 1, 2001; 18(6): 1057 - 1069. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Klappenbach, P. R. Saxman, J. R. Cole, and T. M. Schmidt rrndb: the Ribosomal RNA Operon Copy Number Database Nucleic Acids Res., January 1, 2001; 29(1): 181 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zahn, M. Inui, and H. Yukawa Divergent mechanisms of 5' 23S rRNA IVS processing in the {alpha}-proteobacteria Nucleic Acids Res., December 1, 2000; 28(23): 4623 - 4633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Evguenieva-Hackenberg and G. Klug RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria J. Bacteriol., September 1, 2000; 182(17): 4719 - 4729. [Abstract] [Full Text]
|
|
|
|
|
|
H. A. Addlesee, L. Fiedor, and C. N. Hunter Physical Mapping of bchG, orf427, and orf177 in the Photosynthesis Gene Cluster of Rhodobacter sphaeroides: Functional Assignment of the Bacteriochlorophyll Synthetase Gene J. Bacteriol., June 1, 2000; 182(11): 3175 - 3182. [Abstract] [Full Text]
|
|
|
|
|
|
M. Choudhary and S. Kaplan DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T Nucleic Acids Res., February 15, 2000; 28(4): 862 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mackenzie, A. E. Simmons, and S. Kaplan Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1 Genetics, October 1, 1999; 153(2): 525 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Nereng and S. Kaplan Genomic Complexity among Strains of the Facultative Photoheterotrophic Bacterium Rhodobacter sphaeroides J. Bacteriol., March 1, 1999; 181(5): 1684 - 1688. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. A. Yeliseev, K. E. Krueger, and S. Kaplan A mammalian mitochondrial drug receptor functions as a bacterial "oxygen" sensor PNAS, May 13, 1997; 94(10): 5101 - 5106. [Abstract] [Full Text] [PDF]
|
|