Skip Navigation

This Article
Right arrow Print PDF (1053K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (100)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mills, F. C.
Right arrow Articles by Camerini-Otero, R.D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mills, F. C.
Right arrow Articles by Camerini-Otero, R.D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 24 7305-7316
© 1990

Articles

Sequences of human immunoglobulin switch regions: implications for recombination and transcription

Frederick C. Mills, Jeffrey S. Brooker and R.Daniel Camerini-Otero*

Genetics and Biochemistry Branch, NIDDK Building 10, Room 9D15, NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received September 19, 1990. Revised November 12, 1990. Accepted November 12, 1990.

We have sequenced the entire human Sµ and S-{gamma}4 immunoglobulin heavy chain class switch regions, and have also completed the sequence of human S{varepsilon}. Sµ is composed predominantly of GAGCT and GGGCT pentameric repeats, with these units also being found in S{varepsilon} at a much tower density. Sµ-S{gamma}4 matches are infrequent, but S{gamma}4 contains a cluster of repeated sequences similar to units in mouse {gamma} switch sites and unrelated to the Sµ repeats, suggesting that Sµ-S{gamma} homology is not important in µ-{gamma} switching. We examined our {varepsilon} and {gamma}4 sequences for features that could regulate production of ‘sterile’ transcripts preceding switch recombination. There is an Evolutionarily Conserved Sequence (ECS) upstream from the human and mouse S{varepsilon} regions that overlaps and extends 5' to the start sites for human and mouse {varepsilon} sterile transcripts. Similarly, an ECS upstream from S{gamma}4 is homologous to a mouse sequence that overlaps and extends 5' to the start sites for mouse {gamma}2b sterile transcripts. The {varepsilon} and {gamma}4 conserved segments contain potential interferon Stimulable Response Elements (ISRE's) that are identical between human {varepsilon}and {gamma}4.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
X. Yang, K. Lee, J. Said, X. Gong, and K. Zhang
Association of Ig/BCL6 translocations with germinal center B lymphocytes in human lymphoid tissues: implications for malignant transformation
Blood, September 15, 2006; 108(6): 2006 - 2012.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. L. Wang and M. Wabl
DNA Acrobats of the Ig Class Switch
J. Immunol., May 15, 2004; 172(10): 5815 - 5821.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Schaffer, E. C. Kim, X. Wu, H. Zan, L. Testoni, S. Salamon, A. Cerutti, and P. Casali
Selective Inhibition of Class Switching to IgG and IgE by Recruitment of the HoxC4 and Oct-1 Homeodomain Proteins and Ku70/Ku86 to Newly Identified ATTT cis-Elements
J. Biol. Chem., June 13, 2003; 278(25): 23141 - 23150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. VERCELLI
One Cytokine, Two Isotypes . A Trojan Horse, Pandora's Box, and an Evolving Paradigm
Am. J. Respir. Crit. Care Med., September 1, 2000; 162(3): S86 - 90.
[Full Text] [PDF]

Home page
 Int ImmunolHome page
H. Kitao, H. Arakawa, K.-i. Kuma, H. Yamagishi, N. Nakamura, S. Furusawa, H. Matsuda, M. Yasuda, S. Ekino, and A. Shimizu
Class switch recombination of the chicken IgH chain genes: implications for the primordial switch region repeats
Int. Immunol., July 1, 2000; 12(7): 959 - 968.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. B. Tracy, C. Hsieh, and M. R. Lieber
Stable RNA/DNA Hybrids in the Mammalian Genome: Inducible Intermediates in Immunoglobulin Class Switch Recombination
Science, May 12, 2000; 288(5468): 1058 - 1061.
[Abstract] [Full Text]

Home page
 Genome Res.Home page
E. E. Eichler, N. Archidiacono, and M. Rocchi
CAGGG Repeats and the Pericentromeric Duplication of the Hominoid Genome
Genome Res., November 1, 1999; 9(11): 1048 - 1058.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. N. Weitzmann, K. J. Woodford, and K. Usdin
The Mouse Ms6-hm Hypervariable Microsatellite Forms a Hairpin and Two Unusual Tetraplexes
J. Biol. Chem., November 13, 1998; 273(46): 30742 - 30749.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Fujieda, D. Diaz-Sanchez, and A. Saxon
Combined Nasal Challenge with Diesel Exhaust Particles and Allergen Induces In Vivo IgE Isotype Switching
Am. J. Respir. Cell Mol. Biol., September 1, 1998; 19(3): 507 - 512.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
G. S. Ford, C. H. Yin, B. Barnhart, K. Sztam, and L. R. Covey
CD40 Ligand Exerts Differential Effects on the Expression of I{gamma} Transcripts in Subclones of an IgM+ Human B Cell Lymphoma Line
J. Immunol., January 15, 1998; 160(2): 595 - 605.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.