Skip Navigation

This Article
Right arrow Print PDF (5935K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Galcerán, J.
Right arrow Articles by Izquierdo, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galcerán, J.
Right arrow Articles by Izquierdo, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 3 539-545
© 1990

MOLECULAR BIOLOGY

Transcription at the ecdysone-inducible locus 2B5 in Drosophila

Joan Galcerán+, Javier Llanos, Javier Sampedro, Olaf Pongs1 and Marta Izquierdo*

Departamento de Biología Molecular, Universidad Autónoma de Madrid 28049-Madrid, Spain 1Lehrstuhl für Biochemie der Ruhr-Universität Bochum, Universitätsstrasse 150, D-4630 Bochum, FRG +Present address: Institute Cajal CSIC, Dr. Arce 37, 280002-Madrid, Spain

* To whom correspondence should be addressed

Received October 12, 1989. Revised January 5, 1990. Accepted January 5, 1990.

The Broad-Complex (BR-C) of D. melanogaster, mapping at the 2B5 early ecdysone puff, mediates ecdysone-induced processes. We present here the transcriptional analysis of the locus in both wild type and representative mutants. Two well defined regions are transcribed, only one of which has a pattern consistent with the proposed 2B5 located BR-C function. The 2B5 region is actively transcribed in early third instar larvae before ecdysone levels increase. Ecdysone switches this early pattern to a complex late type which requires the presence of functional BR-C product. Therefore, BR-C self-regulates its own ecdysone-induced transcription. The effect of 2B5 mutations on transcription at several intermolt, early and late puffs is also described. Null mutations at the 2B5 locus prevents ecdysone inducible transcription. This evidence supports the fact that 2B5 codes for an ecdysone-dependent transcriptional regulator.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
D. Godt, J. L. Couderc, S. E. Cramton, and F. A. Laski
Pattern formation in the limbs of Drosophila: bric a brac is expressed in both a gradient and a wave-like pattern and is required for specification and proper segmentation of the tarsus
Development, November 1, 1993; 119(3): 799 - 812.
[Abstract] [PDF]

Home page
 DevelopmentHome page
F. Karim, G. Guild, and C. Thummel
The Drosophila Broad-Complex plays a key role in controlling ecdysone-regulated gene expression at the onset of metamorphosis
Development, January 7, 1993; 118(3): 977 - 988.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
F D Karim and C S Thummel
Ecdysone coordinates the timing and amounts of E74A and E74B transcription in Drosophila.
Genes & Dev., June 1, 1991; 5(6): 1067 - 1079.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.