Skip Navigation

This Article
Right arrow Print PDF (816K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Churchill, G. A.
Right arrow Articles by Waterman, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Churchill, G. A.
Right arrow Articles by Waterman, M. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 3 589-597
© 1990

ENZYMOLOGY

The distribution of restriction enzyme sites in Escherichia coli

Gary A. Churchill, Donna L. Daniels1 and Michael S. Waterman

Department of Mathematics, University of Southern California Los Angeles, CA90089, USA 1Laboratory of Genetics, University of Wisconsin Madison, WI 53706, USA

Received September 29, 1989. Revised December 28, 1989. Accepted December 28, 1989.

A statistical analysis of physical map data for eight restriction enzymes covering nearly the entire genome of E. coli is presented. The methods of analysis are based on a top-down modeling approach which requires no knowledge of the statistical properties of the base sequence. For most enzymes, the distribution of mapped sites is found to be fairly homogeneous. Some heterogeneity in the distribution of sites is observed for the enzymes PstI and HindIII. In addition, BamHI sites are found to be more evenly dispersed than we would expect for random placement and we speculate on a possible mechanism. A consistent departure from a uniform distribution, observed for each of the eight enzymes, is found to be due to a lack of closely spaced sites. We conclude from our analysis that this departure can be accounted for by deficiencies in the physical map data rather than non-random placement of actual restriction sites. Estimates of the numbers of sites missing from the map are given, based both on the map data itself and on the site frequencies in a sample of sequenced E. coli DNA. We conclude that 5 to 15% of the mapped sites represent multiple sites in the DNA sequence.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
I. Mruk and R. M. Blumenthal
Real-time kinetics of restriction-modification gene expression after entry into a new host cell
Nucleic Acids Res., May 1, 2008; 36(8): 2581 - 2593.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Pevzner and G. Tesler
Human and mouse genomic sequences reveal extensive breakpoint reuse in mammalian evolution
PNAS, June 24, 2003; 100(13): 7672 - 7677.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S Karlin and V Brendel
Chance and statistical significance in protein and DNA sequence analysis
Science, July 3, 1992; 257(5066): 39 - 49.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.