Skip Navigation

This Article
Right arrow Print PDF (5134K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (31)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wesley, C. S.
Right arrow Articles by Eanes, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wesley, C. S.
Right arrow Articles by Eanes, W. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 3 599-603
© 1990

GENOME STRUCTURE AND MAPPING

Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer

Cedric S. Wesley, Mathew Ben2, Martin Kreitman2, Nabil Hagag1 and Walter F. Eanes

Department of Ecology and Evolution, State University of New York Stony Brook, NY 11794, USA 1Division of Oncology, State University of New York Stony Brook, NY 11794, USA 2Department of Biology, Princeton University Princeton, NJ 08544, USA

Received September 27, 1989. Revised December 12, 1989. Accepted December 12, 1989.

A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling of the amplified DNA using the random primer method, and screening of a standard library with the probe to identify and isolate clones carrying sequences homologous to the dissected region. This procedure has the potential to replace the difficult procedure of microcloning, as well as facilitate chromosome walking.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
S. Wang, J. He, Z. Cui, and S. Li
Self-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking
Appl. Envir. Microbiol., August 1, 2007; 73(15): 5048 - 5051.
[Abstract] [Full Text] [PDF]

Home page
 Neuro OncolHome page
I. E. McCutcheon, S. J. Hentschel, G. N. Fuller, W. Jin, and G. J. Cote
Expression of the splicing regulator polypyrimidine tract-binding protein in normal and neoplastic brain
Neuro-oncol, January 1, 2004; 6(1): 9 - 14.
[Abstract] [PDF]

Home page
 Genome ResHome page
C. D. Herring, C. Chevillard, S. L. Johnston, P. J. Wettstein, and R. Riblet
Vector-Hexamer PCR Isolation of All Insert Ends from a YAC Contig of the Mouse Igh Locus
Genome Res., June 1, 1998; 8(6): 673 - 681.
[Abstract] [Full Text]

Home page
 Genome ResHome page
E D Green and P Green
Sequence-tagged site (STS) content mapping of human chromosomes: theoretical considerations and early experiences.
Genome Res., November 1, 1991; 1(2): 77 - 90.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.