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Nucleic Acids Research, 1990, Vol. 18, No. 3 625-629
© 1990

MOLECULAR BIOLOGY

Detection of a novel minisatellite-specific DNA-binding protein

Andrew Collick and Alec J. Jeffreys

Department of Genetics, University of Leicester, University Road Leicester LE1 7RH, UK

Received September 7, 1989. Revised January 10, 1990. Accepted January 10, 1990.

We describe the detection of a ubiquitous DNA-binding protein which appears to interact specifically with tandem-repeated minisatellites. The murine 40kd protein, which we term Msbp-1, was found to be present in all mouse tissues tested. This protein was bound specifically and with high affinity by double-stranded DNA containing a repeat sequence related to the minisatellite ‘core’ sequence, and binding required the presence of multiple repeat units. Corresponding minisatellite-specific DNA-binding proteins could also be detected in species ranging from Drosophila to man. This analysis represents the first direct evidence that minisatellites can function as a specific recognition signal for an endogenous DNA-binding protein.


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