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Nucleic Acids Research, 1990, Vol. 18, No. 4 753-758
© 1990

MOLECULAR BIOLOGY

Does butyiphenyl-deoxyguanosine triphosphate differentially inhibit DNA polymerase {alpha} and {delta} activities in permeabilized HeLa cells?

A. Jackson, Dean

Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford OX1 3RE, UK

Received November 23, 1989. Revised January 18, 1990. Accepted January 18, 1990.

In eukaryotic cells, two enzymes, DNA polymerases {alpha} and {delta}, are thought to play major roles in DNA synthesis. I have used butylphenyl dGTP (BuPdGTP), a potent inhibitor of purified DNA polymerase {alpha}, to assess the relative activities of these enzymes in two permeabilized cell systems. In both instances BuPdGTP eliminated all of the activity which was sensitive to aphidicolin. However, no conditions were found where BuPdGTP preferentially inhibited the synthesis of Okazaki fragments-the presumed products of DNA polymerase {alpha} activity. This implies that DNA polymerase activities on the two sides of the replication fork are unable to operate independently, being just two elements of the integrated replication machinery that undertakes DNA synthesis In permeabilized cells.


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