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Nucleic Acids Research, 1990, Vol. 18, No. 4 759-761
© 1990

MOLECULAR BIOLOGY

The B – A transition in superhelical DNA

Dmitry Yu. Krylov, Vladimir L. Makarov and Valery l. lvanov

Engelhardt Institute of Molecular Biology, USSR Academy of Sciences Vavilov Street 32, Moscow 117984, USSR

Received November 23, 1989. Accepted December 20, 1989.

Relaxation of a DNA superheilcal stress due to the B to A transition induced by trifluoroethanol has been studied by assessing the change of DNA orientation in a flow gradient. Using DNAs of different superhelical densities, a decrease in the winding angle during the B-A shift of DNA was found to be 1.5° per base pair in solution. Accepting the winding angle for B-DNA in solution to be 34.1°, that for A-DNA must have a value of 32.6° which agrees with the X-ray data for A-DNA in the condensed state. The date obtained within the B - A transition interval make It possible to conclude that there is an increase in winding at each B/A junction, which is about 5° per one junction.


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