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Nucleic Acids Research, 1990, Vol. 18, No. 4 785-792
© 1990

MOLECULAR BIOLOGY

SLACS retrotransposon from Trypanosoma brucei gambiense is similar to mammalian LINEs

Serap Aksoy1,2,*, Suzanne Williams1, Sandy Chang+ and Frank F. Richards1,2

1Yale MacArthur Center for Molecular Parasitology Yale University School of Medicine New Haven, CT 06510, USA 2Department of Internal Medicine, Yale University School of Medicine New Haven, CT 06510, USA

*To whom correspondence should be addressed

Received November 17, 1989. Revised January 11, 1990. Accepted January 11, 1990.

We have characterized a retrotransposon in Trypanosoma brucei gambiense uniquely associated with the spliced-leader (SL) RNA gene cluster (Spliced Leader Associated Conserved Sequence, SLACS). There are nine copies of SLACS and DNA sequence analysis of one shows the hallmarks of Line-1 like elements. SLACS has generated a 49 bp target DNA duplication at its insertion site and its 3'-end is preceeded by a poly(A) stretch. Two putative open reading frames (ORFs) span 75% of the element. ORF1 has CysHis motif associated with the retroviral gag polypeptide while ORF2 shows homology with reverse transcriptase sequences. Its 5'-end contains a repeated segment of a 185 bp that varies in copy number in different SLACS insertions. Retrotransposon-like sequences inserted into the SL-RNA genes occur in several hemoflagellates. These elements may represent a related family which has maintained its target site specificity.

+Present address: Rockefeller University, 1230 York Ave, New York, NY 10021, USA


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