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Nucleic Acids Research, 1990, Vol. 18, No. 4 837-844
© 1990

MOLECULAR BIOLOGY

The methylation of one specific guanosine in a pre-tRNA prevents cleavage by RNase P and by the catalytic M1 RNA

Dietmar Kahle, Uta Wehmeyer, Shoba Char1 and Guido Krupp

Institute for General Microbiology, Christian-Albrechts-Universität Am Botanischen Garten. 1-9, D-2300 Kiel, FRG 1Department of Molecular Biophysics and Biochemistry, Yale University PO Box 6666, New Haven, CT 06511, USA

Received November 11, 1989. Revised January 22, 1990. Accepted January 22, 1990.

Several modified nucleosides were introduced during in vitro RNA synthesis into a pre The pre tRNAser were used as substrates for RNase P enzymes. No effects were observed with biotin-8-ATP or [{alpha}-S]-GPT whereas with m7 the cleavage reaction was completely inhibited. Analysis of pre-tRNAs which contained m7 at various positions has revealed a single base at the 5'-end of the acceptor stem where this modification absolutely prevents cleavage by catalytic M1 RNA, eukaryotic and prokaryotic RNase P holoenzymes. These results suggest that a critical contact must be made between pre-tANA substrate and enzyme/ribozyme or that the approach of the potential cleaving agent (a positive magnesiumion) is made impossible by the positive charge at N-7 of the guanosine. in addition, we have shown that a pre-tRNA containing only M7G's can still form a complex with M1 RNA in a gel retardation assay.


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