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Nucleic Acids Research, 1990, Vol. 18, No. 4 845-853
© 1990

MOLECULAR BIOLOGY

Internal eliminated sequenc are removed prior to chromosome fragmentation during development in Euplotes crassus

S.Lorraine Tausta and Lawrence A. Klobutcher

Department of Biochemistry, University of Connecticut Health Center Farmington, CT 06032, USA

Received November 11, 1989. Revised January 9, 1990. Accepted January 9, 1990.

The hypotrichous ciliated protozoa undergo a massive genome rearrangement process after their sexual cycle. One frequent type of rearrangement is the removal of DNA sequences (internal eliminated sequences; IESs) from internal regions of DNA molecules. In this study, we characterized the removal of IESs in Euplotes crassus. Southern hybridization analyses combined with cytological observations Indicated that IES removal is an early event in macronuclear development, occurring during the polytene chromosome stage and prior to the chromosome fragmentation process. The results are consistent with IES removal occurring via an intramolecular DNA breakage and rejoining process.


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