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Nucleic Acids Research, 1990, Vol. 18, No. 4 885-890
© 1990

MOLECULAR BIOLOGY

Preservation of a complex satellite DNA in two species of Echinoderms

J. Sainz and L. Cornudella

Unidad de Química Macromolecular del CSIC Universitat Politécnica de Catalunya Av, Diagonal 647, 08028 Barcelona, Spain

Received November 7, 1989. Revised January 3, 1990. Accepted January 3, 1990.

The cloning and sequencing of a tandemly arrayed repetitive DNA sequence from the sea cucumber Holothuria tubulosa has been recently described (Sainz, J., Azorín, F. and Comudella, L. 1989. Gene 80, 57 – 64). We have now searched the genomes of several echinoderm species for the presence of homologous repetitive elements. A close but not identical repeated sequence has been Identified in a related holothuroid, H. polii. The monomeric repeat unit is 391 bp long and has a base composition of 66.8% A and T residues, lined up in tracts of 4 nt or larger. The monomeric sequence lacks any internal subrepeat organization although it displays a substantial degree of internal redundancy in the form of inverted and direct repeats. The repeated element accounts for 0.34% of the genome which corresponds to a repetition frequency of about 0.5 x 105 copies per haploid complement. The intra- and interspecific homologies among monomers of the satellite DNA as derived from sequence analyses are very high, averaging 97%. The results suggest that the homogeneity of the highly reiterated DNA sequence may be attributed to evolutionary conservative trends.


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