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Nucleic Acids Research, 1990, Vol. 18, No. 4 989-992
© 1990

GENOME STRUCTURE AND MAPPING

Electrophoretic behavior of d(GGAAAAAAGG)n, d(CCAAAAAACC)n, and (CCAAAAAAGG)n, and implications for a DNA bending model

R.A. Abagyan*, V.N. Mironov, B.K. Chernov, V.P. Chuprina1 and A.V. Ulyanov

Engelhardt Institute of Molecular Biology USSR Academy of Sciences, Moscow B-334, 117984 1Research Computer Center USSR Academy of Sciences, Pushchino, Moscow region, USSR

To whom correspondence should be addressed at Unversité de Liege, Laboratoire de Genie Genetique, Institut de Chimie B6, B 4000 Sart Tilman, Belgium

Received September 25, 1989. Revised December 15, 1989. Accepted December 15, 1989.

Double stranded multimers and (C2A6C2)n,(C2A6G2)n, and (C2A6G2)n, were prepared from chemically synthesized oligonucleotldes to study the influence of sequences flanking the An tract on the curvature of DNA. All these duplexes, including polypurine . polypyrimidine one, exhibit strong retardation in polyacrylamide gel which is indicative of pronounced DNA curvature. ft has been proposed previously that among the bends at the boundary with the ollgo(A) tract two types should be distinguished: 5'-bends and 3'-bends (Koo et al., 1986) This distinction was deduced from different relative mobilities of two specially designed sequences having phased 5'-bends and 3'-bends, respectively. Our data indicate that the substitutions of nucleotides at both 5' and 3' boundaries of A6 tract result in comparable changes in relative mobility. Therefore, for B-B' bends it is important to take Into account not only whether they are at the 5' or 3' end of an ollgo(dA) tract, but also the particular sequences at the boundaries of this tract.


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