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Nucleic Acids Research, 1990, Vol. 18, No. 5 1115-1119
© 1990

MOLECULAR BIOLOGY

The cyanelle genome of Cyanophora paradoxa, unlike the chloroplast genome, codes for the ribosomal L3 protein

Jean-Luc Evrard, Clayton Johnson1, Ines Janssen2, Wolfgang Löffelhardt2, Jacques-Henry Weil and Marcel Kuntz*

Institut de Biologie Moléculaire des Plantes et Université Louis Pasteur 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France 1Max-Planck-Institut fur Molekulare Genetik Abt. Wittmann, Ihnestrasse 73, 1000 Berlin 33, FRG 2Institut für Allgemeine Biochemie, Universität Wien Währingerstrasse 38, 1090 Wien, Austria

*To whom correspondence should be addressed

Received December 20, 1989. Revised January 30, 1990. Accepted January 30, 1990.

We describe a 1132 bp sequence of the cyanelle genome of Cyanophora paradoxa containing the rpl3 gene. This gene, which is not chloroplast encoded in plants, is the first of a long cyanelle ribosomal operon whose organization resembles that of the S10 operon of E. coli. We have shown that the rpl3 gene is transcribed in cyanelles as a 7500 nucleotide precursor and that the 5-end of the mRNA starts approximately 90 nucleotides upstream from the initiation codon. However, no typical procaryotic promoter could be found for this gene. We have detected, using anti E. coli L3 antibodies, the cyanelle L3 protein in cyanelle extracts and in E. coll cells transformed with the cyanelle rpl3 gene.


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