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Nucleic Acids Research, 1990, Vol. 18, No. 5 1121-1127
© 1990

MOLECULAR BIOLOGY

The human minisatellite consensus at breakpoints of oncogene translocations

Anna Krowczynska1,2,+, Richard A. Rudders1 and G. Krontiris1,2,*

1department of Medicine (Hematology/Oncology) New England Medical Center Hospitals, Boston, MA 02111 2department of Molecular Biology and Microbiology, Tufts University School of Medicine Boston, MA 02111, USA

*To whom correspondence should be addressed

Received April 18, 1989. Revised December 18, 1989. Accepted December 18, 1989.

A reexamination of human minisatellite (hypervariable) regions following the cloning and sequencing of the new minisatellite, VTR1.1, revealed that many of these structures possessed a strongly conserved copy of the chi-like octamer, GC[A/T]GG[A/T]GG. In oncogene translocations apparently created by aberrant VDJ recomblnase activity, this VTR octamer was often found within a few bases of the breakpoint (p < 10–10). Three bcl2 rearrangements which occurred within 2 bp of one another were located precisely adjacent to this consensus; it defined the 5' border of that oncogene's major breakpoint cluster. Several c-myc translocations also occurred within 2 bp of this sequence. While the appearance of a chi-like element in polymorphic minisatellite sequences is consistent with a role promoting either recombination or replication slippage, the existence of such elements at sites of somatic translocations suggests chi function in site-specific recombination, perhaps as a subsidiary recognition signal in immunoglobulin gene rearrangement. We discuss the implications of these observations for mechanisms by which oncogene translocations and minisatellite sequences are generated.

+Present address Department of Biochemistry, Tufts University School of Medicine


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