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Nucleic Acids Research, 1990, Vol. 18, No. 5 1129-1132
© 1990

GENOME STRUCTURE AND MAPPING

Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates

Y. Kashi, Y. Tikochinsky, E. Genislav, F. lraqi, A. Nave, J.S. Beckmann1, Y. Gruenbaum and M. Soller

Department of Genetics, The Life Sciences Institute, The Hebrew University of Jerusalem 91904, Jerusalem 1Department of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center 50250 Bet Dagan, Israel

Received December 14, 1989. Revised February 6, 1990. Accepted February 6, 1990.

Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA ‘fingerprint’ patterns were obtained in chicken, sheep, and horse, moderately variable DNA ‘fingerprints’ in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poty-TG-containing probes can uncover useful genetic variation.


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