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Nucleic Acids Research, 1990, Vol. 18, No. 5 1133-1136
© 1990

GENOME STRUCTURE AND MAPPING

Circular chromosomal DNA in the sulfur-dependent archaebacterium Sulfolobus acidocaldarius

Akihiko Yamagishi and Tairo Oshima

Department of Life Science, Tokyo Institute of Technology Nagatsuta, Yokohama 227, Japan

Received December 14, 1989. Revised February 6, 1990. Accepted February 6, 1990.

The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acldocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acldocaldarius chromosomal DNA. It contained two Notl sites. Both 1.0 and 2.1 Mbp DNA band separated by PFGE were hybridized with the two Independent Notl-linking fragment. Each right and left arms of two Notl-linking fragments were hybridized wfth one of the two DNA bands separated by PFGE. The results indicated that the chromosomal DNA of S.acldocaldarius is circular.


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