Nucleic Acids Research, 1990, Vol. 18, No. 6 1369-1376
© 1990
MOLECULAR BIOLOGY |
The nuclear SUV3-1 mutation affects a variety of post-transcriptional processes in yeast mitochondria
1Department of Molecular Genetics, The Ohio State University Columbus, OH 43210–1292 2Department of Biochemistry, The University of Texas Southwestern Medical Center Dallas, TX 75235-9038, USA
*To whom correspondence should be addressed
Received January 18, 1990. Accepted February 7, 1990.
The SUV3-1 mutation was isolated earlier as a suppressor of a deletion of a conserved RNA processing site (dodecamer) near the 3' end of the var1 gene. Previous studies Indicate that the suppressor enhances translation of mutant var1 messages; unexpectedly, It also causes over-accumulation of excised intron RNA of the large rRNA gene intron and blocks cleavage at the dodecamer site within that intron. In this study most mitochondrial genes in SUV3-1 and suv3 nuclear contexts are surveyed for changes in levels of mRNA, for Interference with dodecamer cleavage and splicing and for levels of excised intron RNAs. SUV3-1 has little or no effect on the size or abundance of unspliced RNAs tested. It results, however, In a marked Increase In the abundance of seven of eight excised group I intron RNAs tested, most of which are not detectable In wild-type (suv3) strains. The suppressor lowers levels of the cob and coxl mRNAs about 2–5 and 20-fold, respectively. The effect on coxi mRNA results from a decrease in the splicing of its intron 5ß Despite the reduction in these mRNA levels, the amounts of coxi and cyt b polypeptldes were close to wild-type levels in SUV3-1 cells. These data show that the suv3 gene plays a prominent role in post-transcriptional and translation events in yeast mitochondria.
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