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Nucleic Acids Research, 1990, Vol. 18, No. 6 1549-1557
© 1990

MOLECULAR BIOLOGY

Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes

David A. Stein, Bradley R. Cairns+ and Scott M. Landfear*

Department of Microbiobgy and Immunology and Vollum Institute for Advanced Biomedical Research, The Oregon Health Sciences University 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA

*To whom correspondence should be addressed

Received November 14, 1989. Revised February 8, 1990. Accepted February 8, 1990.

We have previously cloned a gene for a developmentally regulated transport protein from the trypanosomatid protozoan Leishmania enriettil. We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit. Transcriptional mapping defines a contiguous 3.3 kilobase region of the repeat unit that encodes the mRNA. The 5' end of the mature mRNA contains the spliced leader or mlni-exon previously identified in kinetoplastid protozoa, while the 3' ends of the mRNA are heterogeneous in sequence and in location of the polyadenylation site. We have identified genomic restriction fragments that flank the tandem repeat on the 5' and 3' sides and which may be linked to sequences required for expression of the gene family. Other species of Leishmanla also contain sequences that hybridize to the cloned L. enriettil gene at high stringency.

+ Present address Department of Biochemistiy, Stanford University, Stanford, CA 94305, USA


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