Nucleic Acids Research, 1990, Vol. 18, No. 7 1687-1691
© 1990
CHEMISTRY |
DNA recombination during PCR
Laboratoire de Biologie et Immunologie Moléculaires des Rétrovirus, Institut Pasteur 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
* To whom correspondence should be addressed
Received March 1, 1990. Accepted March 9, 1990.
PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase In Taq DNA polymerase elongatlon time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Blow, T. Zhang, T. Woyke, C. F. Speller, A. Krivoshapkin, D. Y. Yang, A. Derevianko, and E. M. Rubin Identification of ancient remains through genomic sequencing Genome Res., August 1, 2008; 18(8): 1347 - 1353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. F. Keele, E. E. Giorgi, J. F. Salazar-Gonzalez, J. M. Decker, K. T. Pham, M. G. Salazar, C. Sun, T. Grayson, S. Wang, H. Li, et al. Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection PNAS, May 27, 2008; 105(21): 7552 - 7557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Salazar-Gonzalez, E. Bailes, K. T. Pham, M. G. Salazar, M. B. Guffey, B. F. Keele, C. A. Derdeyn, P. Farmer, E. Hunter, S. Allen, et al. Deciphering Human Immunodeficiency Virus Type 1 Transmission and Early Envelope Diversification by Single-Genome Amplification and Sequencing J. Virol., April 15, 2008; 82(8): 3952 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nora, C. Charpentier, O. Tenaillon, C. Hoede, F. Clavel, and A. J. Hance Contribution of Recombination to the Evolution of Human Immunodeficiency Viruses Expressing Resistance to Antiretroviral Treatment J. Virol., July 15, 2007; 81(14): 7620 - 7628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ban, C. Honda, Y. Hatsuyama, M. Igarashi, H. Bessho, and T. Moriguchi Isolation and Functional Analysis of a MYB Transcription Factor Gene that is a Key Regulator for the Development of Red Coloration in Apple Skin Plant Cell Physiol., July 1, 2007; 48(7): 958 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pisoni, G. Bertoni, M. Puricelli, M. Maccalli, and P. Moroni Demonstration of Coinfection with and Recombination by Caprine Arthritis-Encephalitis Virus and Maedi-Visna Virus in Naturally Infected Goats J. Virol., May 15, 2007; 81(10): 4948 - 4955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Hayward, J. Taylor, and A. G. Rodrigo Phylogenetic Analysis of Feline Immunodeficiency Virus in Feral and Companion Domestic Cats of New Zealand J. Virol., March 15, 2007; 81(6): 2999 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Beser, P. Hagblom, and V. Fernandez Frequent In Vitro Recombination in Internal Transcribed Spacers 1 and 2 during Genotyping of Pneumocystis jirovecii J. Clin. Microbiol., March 1, 2007; 45(3): 881 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gao, O. V. Nainan, Y. Khudyakov, J. Li, Y. Hong, A. C. Gonzales, J. Spelbring, and H. S. Margolis Recombinant hepatitis C virus in experimentally infected chimpanzees J. Gen. Virol., January 1, 2007; 88(1): 143 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Suspene, C. Rusniok, J.-P. Vartanian, and S. Wain-Hobson Twin gradients in APOBEC3 edited HIV-1 DNA reflect the dynamics of lentiviral replication Nucleic Acids Res., October 18, 2006; 34(17): 4677 - 4684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Butler, P. Weber, and N. Wertz Antibody Repertoire Development in Fetal and Neonatal Piglets. XIII. Hybrid VH Genes and the Preimmune Repertoire Revisited J. Immunol., October 15, 2006; 177(8): 5459 - 5470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Schienman, R. A. Holt, M. R. Auerbach, and C.-B. Stewart Duplication and Divergence of 2 Distinct Pancreatic Ribonuclease Genes in Leaf-Eating African and Asian Colobine Monkeys Mol. Biol. Evol., August 1, 2006; 23(8): 1465 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Charpentier, T. Nora, O. Tenaillon, F. Clavel, and A. J. Hance Extensive Recombination among Human Immunodeficiency Virus Type 1 Quasispecies Makes an Important Contribution to Viral Diversity in Individual Patients J. Virol., March 1, 2006; 80(5): 2472 - 2482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Markmann and D. Tautz Reverse taxonomy: an approach towards determining the diversity of meiobenthic organisms based on ribosomal RNA signature sequences Phil Trans R Soc B, October 29, 2005; 360(1462): 1917 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Muller, S. C. Stebel, S. Knall, G. Zipf, H. S. Bernauer, and K. M. Arndt Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution Nucleic Acids Res., August 1, 2005; 33(13): e117 - e117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Rozak and P. N. Bryan Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains Nucleic Acids Res., May 18, 2005; 33(9): e82 - e82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, D. M. Netski, Q. Mao, O. Laeyendecker, J. R. Ticehurst, X.-H. Wang, D. L. Thomas, and S. C. Ray Accurate Representation of the Hepatitis C Virus Quasispecies in 5.2-Kilobase Amplicons J. Clin. Microbiol., September 1, 2004; 42(9): 4223 - 4229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Shriner, A. G. Rodrigo, D. C. Nickle, and J. I. Mullins Pervasive Genomic Recombination of HIV-1 in Vivo Genetics, August 1, 2004; 167(4): 1573 - 1583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bello, C. Casado, S. Garcia, C. Rodriguez, J. del Romero, and C. Lopez-Galindez Co-existence of recent and ancestral nucleotide sequences in viral quasispecies of human immunodeficiency virus type 1 patients J. Gen. Virol., February 1, 2004; 85(2): 399 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Chan, Z. B. Kurago, C. A. Stewart, M. J. Wilson, M. P. Martin, B. E. Mace, M. Carrington, J. Trowsdale, and C. T. Lutz DNA Methylation Maintains Allele-specific KIR Gene Expression in Human Natural Killer Cells J. Exp. Med., January 20, 2003; 197(2): 245 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Vartanian and S. Wain-Hobson Analysis of a library of macaque nuclear mitochondrial sequences confirms macaque origin of divergent sequences from old oral polio vaccine samples PNAS, May 28, 2002; 99(11): 7566 - 7569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Sakuragi, A. Iwamoto, and T. Shioda Dissociation of Genome Dimerization from Packaging Functions and Virion Maturation of Human Immunodeficiency Virus Type 1 J. Virol., February 1, 2002; 76(3): 959 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Curran, C. L. Jameson, J. K. Craggs, A. M. Grabowska, B. J. Thomson, A. Robins, W. L. Irving, and J. K. Ball Evolutionary trends of the first hypervariable region of the hepatitis C virus E2 protein in individuals with differing liver disease severity J. Gen. Virol., January 1, 2002; 83(1): 11 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sala, J.-P. Vartanian, P. Kousignian, J.-F. Delfraissy, Y. Taoufik, S. Wain-Hobson, and J. Gasnault Progressive multifocal leukoencephalopathy in human immunodeficiency virus type 1-infected patients: absence of correlation between JC virus neurovirulence and polymorphisms in the transcriptional control region and the major capsid protein loci J. Gen. Virol., April 1, 2001; 82(4): 899 - 907. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Mortreux, I. Leclercq, A.-S. Gabet, A. Leroy, E. Westhof, A. Gessain, S. Wain-Hobson, and E. Wattel Somatic Mutation in Human T-Cell Leukemia Virus Type 1 Provirus and Flanking Cellular Sequences During Clonal Expansion In Vivo J Natl Cancer Inst, March 7, 2001; 93(5): 367 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Qiu, L. Wu, H. Huang, P. E. McDonel, A. V. Palumbo, J. M. Tiedje, and J. Zhou Evaluation of PCR-Generated Chimeras, Mutations, and Heteroduplexes with 16S rRNA Gene-Based Cloning Appl. Envir. Microbiol., February 1, 2001; 67(2): 880 - 887. [Abstract] [Full Text]
|
|
|
|
|
|
S. Gratton, R. Cheynier, M.-J. Dumaurier, E. Oksenhendler, and S. Wain-Hobson Highly restricted spread of HIV-1 and multiply infected cells within splenic germinal centers PNAS, December 19, 2000; 97(26): 14566 - 14571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Becker, P. Böger, R. Oehlmann, and A. Ernst PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities Appl. Envir. Microbiol., November 1, 2000; 66(11): 4945 - 4953. [Abstract] [Full Text]
|
|
|
|
|
|
D. Faulhammer, A. R. Cukras, R. J. Lipton, and L. F. Landweber From the Cover: Molecular computation: RNA solutions to chess problems PNAS, February 15, 2000; 97(4): 1385 - 1389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Romstad, P. Guldberg, N. Blau, and F. Guttler Single-Step Mutation Scanning of the 6-Pyruvoyltetrahydropterin Synthase Gene in Patients with Hyperphenylalaninemia Clin. Chem., December 1, 1999; 45(12): 2102 - 2108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Haber, U Meyer-Konig, and F. Hufert Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination J. Gen. Virol., June 1, 1999; 80(6): 1495 - 1500. [Abstract]
|
|
|
|
|
|
J. Martinez-Picado, A. V. Savara, L. Sutton, and R. T. D'Aquila Replicative Fitness of Protease Inhibitor-Resistant Mutants of Human Immunodeficiency Virus Type 1 J. Virol., May 1, 1999; 73(5): 3744 - 3752. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. G. Brooks, A. H. Filipovich, J. W. Padgett, R. Mamlock, and R. M. Goldblum T-Cell Receptor Analysis in Omenn's Syndrome: Evidence for Defects in Gene Rearrangement and Assembly Blood, January 1, 1999; 93(1): 242 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. McAllister, C. Casino, F. Davidson, J. Power, E. Lawlor, P. L. Yap, P. Simmonds, and D. B. Smith Long-Term Evolution of the Hypervariable Region of Hepatitis C Virus in a Common-Source-Infected Cohort J. Virol., June 1, 1998; 72(6): 4893 - 4905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. French, T. F. McElwain, T. C. McGuire, and G. H. Palmer Expression of Anaplasma marginale Major Surface Protein 2 Variants during Persistent Cyclic Rickettsemia Infect. Immun., March 1, 1998; 66(3): 1200 - 1207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Delwart, H. Pan, A. Neumann, and M. Markowitz Rapid, Transient Changes at the env Locus of Plasma Human Immunodeficiency Virus Type 1 Populations during the Emergence of Protease Inhibitor Resistance J. Virol., March 1, 1998; 72(3): 2416 - 2421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W Ji, X Y Zhang, G S Warshamana, G Z Qu, and M Ehrlich Effect of internal direct and inverted Alu repeat sequences on PCR. Genome Res., October 1, 1994; 4(2): 109 - 116. [Abstract] [PDF]
|
|
|
|
|
|
S J Odelberg and R White A method for accurate amplification of polymorphic CA-repeat sequences. Genome Res., August 1, 1993; 3(1): 7 - 12. [Abstract] [PDF]
|
|
|
|
|
|
I S Bevan, R Rapley, and M R Walker Sequencing of PCR-amplified DNA. Genome Res., May 1, 1992; 1(4): 222 - 228. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Erlich, D Gelfand, and J. Sninsky Recent advances in the polymerase chain reaction Science, June 21, 1991; 252(5013): 1643 - 1651. [Abstract] [PDF]
|
|
|
|
|
|
S Wain-Hobson, J. Vartanian, M Henry, N Chenciner, R Cheynier, S Delassus, L. Martins, M Sala, M. Nugeyre, D Guetard, et al. LAV revisited: origins of the early HIV-1 isolates from Institut Pasteur Science, May 17, 1991; 252(5008): 961 - 965. [Abstract] [PDF]
|
|