Nucleic Acids Research, 1990, Vol. 18, No. 7 1725-1729
© 1990
MOLECULAR BIOLOGY |
Programmed ribosomal frameshifting generates the Escherichia coli DNA polymerase III 
subunit from within the 
subunit reading frame
Department of Microbiology, The Universrty of Texas Austin, TX 78712, USA
Received January 5, 1990. Revised February 22, 1990. Accepted February 22, 1990.
The Escherichia coli dnaX gene encodes both the 
and 
subunits of DNA polymerase III holoenzyme in one reading frame. The 71.1 kDa and the shorter share N-terminal sequences. Mutagenesis of a potential ribosomal frameshift signal located at codons 428–430 without changing the amino acid sequence of the product, eliminated detectable synthesis of the subunit, suggesting that the reading frame is shifted at that sequence and is terminated by a nonsense codon located in the –1 frame 3 nucleotides downstream of the signal. This seems to be the first known case of a frameshift which is used, along with the termination codon in the –1 frame, to terminate a peptide within a reading frame. [Mutagenesis of a dibasic peptide (lys-lys) at codons 498–499, the site at which a Lacz fusion protein was cleaved in vitro (1) had no effect on formation in vivo, suggesting that cleavage observed in vitro is not the mechanism of formation in vivo.]
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