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Nucleic Acids Research, 1990, Vol. 18, No. 7 1763-1769
© 1990

MOLECULAR BIOLOGY

The stability, toxicity and effectiveness of unmodified and phosphorothioate antisense oligodeoxynucleotides in Xenopus oocytes and embryos

Tod M. Woolf, Charles G.B. Jennings, Michael Rebagliati1 and Douglas A. Melton

Department of Biochemistry and Molecular Biology, Harvard University 7 Divinity Avenue, Cambridge, MA 02138 1Department of Zoology, University of Texas at Austin Austin, TX 78712, USA

Received December 12, 1989. Revised March 6, 1990. Accepted March 6, 1990.

The properties of antisense phosphorothioate and unmodified oligodeoxynucleotides have been studied in Xenopus oocytes and embryos. We find that phosphorothioates, like unmodified oligodeoxynucieotides, can degrade Vg1 mRNA in oocytes via an endogenous RNase H-like activity. in oocytes, phosphorothioate oligodeoxynucleotides are more stable than unmodified oligoeoxynucleotides and are more effective in degrading Vg1 mRNA. In embryos, neither unmodified nor phosphorothioate deoxyoligonuleotides were effective in degrading Vg1 message at sub-toxic doses.


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