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Nucleic Acids Research, 1990, Vol. 18, No. 7 1783-1787
© 1990

MOLECULAR BIOLOGY

Properties of the transcriptional enhancer in Saccharomyces cerevisiae telomeres

Kurt W. Runge and Virginia A. Zakian

Division of Basic Sciences, M621, Fred Hutchinson Cancer Research Center 1124 Columbia Street, Seattle, WA 98104, USA

Received December 6, 1989. Revised February 21, 1990. Accepted February 21, 1990.

Saccharomyces cerevisiae chromosomes end wtth the sequence C2–3A(CA)1–4, commonly abbreviated as C1–3A. These sequences can function as upstream activators of transcription (UAS's) when placed in front of a CYC1-lacZ fusion gene. When C1–3 sequences are placed between the GAL1,10 UAS and the CYC1-lacZ fusion, the C1–3A UAS still functions and the amount of ß-galactosidase produced in cells grown on glucose is as much or more than that for cells grown on either glycerol medium, or cells grown on glucose medium containing a plasmid with just the C1–3A UAS. These data indicate that the UAS is immune from glucose repression from the upstream GAL 1,10 UAS. Because C1–3A sequences are bound in vitro by the transcription factor RAP1, the UAS activity of yeast telomere sequences was compared with that of a similar UAS from the tightly regulated ribosomal protein gene RP39A, which also contains a RAP1 binding site. While transcription from the ribosomal protein gene UAS was responsive to cell density, the amount of transcription from the C1–3A UAS was nearly the same at all cell densities tested. These data show that the transcriptional activation by C1–3A sequences is not regulated by cell density.


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