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Nucleic Acids Research, 1990, Vol. 18, No. 7 1853-1858
© 1990

MOLECULAR BIOLOGY

Transcriptional stimulation via SC site of Bombyx sericin-1 gene through an interaction with a DNA binding protein SGF-3

Kenji Matsuno, Shigeharu Takiya, Chi-Chung Hui, Tishiharu Suzuki, Masakazu Fakuta, Kohji Ueno and Yoshiaki Suzuki

Department of Developmental Biology, National Institute for Basic Biology Myodaiji, Okazaki 444, Japan

Received October 4, 1989. Revised January 3, 1990. Accepted January 3, 1990.

Three protein binding sites have been identified in the upstream region of the sericin-1 gene. Two of them, SA and SC sites, have been known as putative cis-acting elements. Using synthetic oligonucleotides of these binding sites, it was found that silk gland factor-1 (SGF-1) binds to the SA site, and silk gland factor-3 (SGF-3) binds to the SC site but not to a mutated SC site, SCM. Tissue distribution of the two factors was different. SGF-3 is present abundantly in the middle silk gland (MSG) where the sericin-1 gene is transcribed specifically but is also present in other cell types, though in a much less concentration. SGF-1 is observed very abundantly in the two parts of silk gland, MSG and posterior silk gland (PSG), but not in other cells. Templates containing muitimerized SA or SC sites at –39 of the sericin-1 gene promoter were tested in MSG nuclear extracts. The SC multimer strongly activated transcription, while the mutant SCM multimer did not. The SA multimer also gave a slight stimulation of transcription. These results suggest that SGF-3 stimulates transcription through an interaction with the SC site, and SGF-1 does so weakly through the SA site.


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