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Nucleic Acids Research, 1990, Vol. 18, No. 8 2061-2064
© 1990

ENZYMOLOGY

Fsel, a new type II restriction endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'

Janise Meyertons Nelson+, Sheila M. Miceli, Mary P. Lechevalier1 and Richard J. Roberts*

Cold Spring Harbor Laboratory Cold Spring Harbor, NY 11724 1Waksman Institute, Rutgers University Piscataway, NJ 08855, USA

* To whom correspondence should be addressed

Received December 28, 1989. Revised February 26, 1990. Accepted February 26, 1990.

A Type II restriction endonuclease, designated Fsel, has been partially purified from a Frankia species (NRRL 18528). This enzyme cleaves Adenovirus 2 DNA at three sites, but does not cleave the DNAs from bacteriophages lambda, T7, and øX174, the animal virus SV40, pUC18 and pBR322. Fsel recognizes the octanucleotide sequence 5' GGCCGGICC 3' and cleaves as indicated by the arrow. The frequency of occurrence of Fsel sites within sequenced regions of the human genome is similar to that for Notl sites.

+ Present address: Abbott Laboratories, Department 451, Building R5, Shendan Road, N. Chicago IL 60064, USA


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