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Nucleic Acids Research, 1990, Vol. 18, No. 8 2065-2068
© 1990

CHEMISTRY

Backbone-modified oligonucleotides containing a butanediol-1,3 moiety as a ‘vicarious segment’ for the deoxyribosyl moiety—synthesis and enzyme studies

Andrzej Wilk, Maria Koziolkiewicz, Andrzej Grajkowski, Bogdan Uznanski and Wojciech J. Stec

Polish Academy of Sciences, Centre of Molecular and Macromolecular Studies, Department of

Received December 21, 1989. Revised March 19, 1990. Accepted March 19, 1990.

Sequential single replacement of nucleosides within the decanucleotide d[GGGAATTCCC] (7) by means of a butanediol-1,3 residue allowed us to obtain a set of ten decanucleotides containing ‘vicarious’ (V) carbonphosphate fragments. These analogues were further used as substrates for svPDE, nuclease PI and EcoR1 endonuclease. Interestingly, replacement of any of the nucleosides within the canonical sequence ...GAATTC... by the butanediol-1,3 ‘vicarious’ segment discriminates such constructs 8c-h as substrates for Eco-R1 enzyme.


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