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Nucleic Acids Research, 1990, Vol. 18, No. 8 2141-2148
© 1990

CHEMISTRY

Stabilization of the Z' form of poly(dGdC):poly(dGdC) in solution by multivalent ions relates to the Z form in crystals

Mark E. Harder and W.Curtis Johnson, Jr

Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331-6503, USA

Received November 20, 1989. Revised March 7, 1990. Accepted March 7, 1990.

Both multivalent ions and 85% ethanol are required to produce the original Z' form of poly(dGdC):poly(dGdC) In solution. When multivalent impurities are removed by dialysis against 0.5 M NaCI and 1 mM EDTA, the circular dichroism retains features of the standard Z form. Addition of Ca+2 nearly reverses this effect. Analysis by singular value decomposition of nearultraviolet circular dichroism spectra collected during titrations of polynucleotide in 60% ethanol with multivalent ions reveal that, at concentrations below .5 per nucleotide, they stabilize Z'-like forms in a two-state equilibrium with the Z form. Differences among the Z' spectra produced by the different ions suggest that at least three families of Z' structures exist. Furthermore, comparison with crystal data indicates that the Z' form in solution is related to the Z form in crystals.


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