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Nucleic Acids Research, 1990, Vol. 18, No. 9 2625-2631
© 1990

Molecular Biology

Light affects the structure of Chlamydomonar Chloroplast chromosomes

Robert J. Thompson and Gisela Mosig

Department of Molecular Biology, Vanderbilt University Nashville, TN 37235, USA

Received February 2, 1990. Accepted March 16, 1990.

We have analyzed changes in the structure of chloroplast chromosomes in response to light in growing Chlamydomonas cells using a crosslinking assay based on the intercalation of HMT (4'-hydroxymethyl-4,5',8-trimethylpsoralen) into DNA. Our results show that the structure of chloroplast chromosomes in at least three widely separated regions is different in light-grown vs. dark-grown cells. Structural changes in chloroplast chromosomes occur within 3 hrs after exposure to light or darkness, respectively. The response to light is not inhibited by atrazine and can be elicited by dim blue light incapable of evolving O2, indicating that it does not require photosynthesis. Inhibition of cytoplasmic protein synthesis with cycloheximide prevents this response to light, indicating that it depends, at least in part, on proteins imported from the cytoplasm.


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