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Nucleic Acids Research, 1990, Vol. 18, No. 9 2685-2689
© 1990

Molecular Biology

A cDNA clone closely associated with non-A, non-B hepatitits

Mitsugo Maèno*, Kazuyoshi Kaminaka, Hiroyuki Sugimoto, Mariko Esumi, Nakanobu Hayshi, Kohei Komatsu, Kenji Abe1, Sadayoshi Sekiguchi2, Michitami Yano3, Kyosuke Mizuno4 and Toshio Shikata

Department of Pathology, Nihon University School of Medicine 30-1 Oyaguchikami-machi, Itabashiku, Tokyo 173 1Department of Pathology, National Institute of Healty Tokyo 2Hokkaido Red Cross Blood Center Sapporo 3Institute for Clinical Research, Nagasaki Chuo National Hospital Nagasaki 4Chemo-sero-therapeutic Research Institute Kumamoto, Japan

*To whom correspondence should be addressed

Received January 16, 1990. Accepted March 28, 1990.

A lambda gtl1 cDNA library was constructed from RNA purified from hepatitis B viral surface antigen-negative human plasma with high alanine aminotransferase activity. A cDNA clone, designated s C8-2, was isolated by immunoscreening with mixed sera from non-A, non-B hepatitis (NANBH) carrier and convalescent chimpanzees. The recombinant protein produced by C8-2 reacted specifically with sera of patients in the chronic phase of NANBH. The sequence of C8-2, 269 bp, did not hybridized with any human or chimpanzee genomic DNA, and had no homology with those of primates and viruses. The existence of this sequence in RNA of possibly infectious plasma was shown by RNA blot hybridization and by Southern blot analysis of products amplified by the polymerase chain reaction. These results strongly suggest that C8-2 is derived from the agent of this viral hepatitis.


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