Nucleic Acids Research, 1990, Vol. 18, No. 9 2721-2731
© 1990
Molecular Biology |
A 46 base pair enhancer sequence within the locus activating region is required for induced expression of the gamma-globin gene during erythroid differentiation
Clinical Hematology Branch, National Heart Lung Blood Institute, National Institutes of Health Bethesda, MD 20892, USA
Received December 14, 1989. Accepted March 28, 1989.
The locus activating region (LAR), contained within 30 kb of chromatln flanking the human beta-globln gene cluster, has recently been shown to be essential for high level beta-globin gene expression. To determine the effect of fragments containing LAR sequences on globin gene expression, mRNA from a marked gammaglobin gene linked to LAR fragments was assayed in stably transfected K562 erythroleukemia cells. DNasel hypersensitive site II (HS II), located 10.9 kb upstream of the epsilon-globin gene, was required for high level gamma-globin gene expression. We also showed that a 46 bp enhancer element within HS II was necessary and sufficient for the increased gamma-globin gene expression observed with hemin induced erythroid maturation of K562 cells. These results localize a distant regulatory element important for activation of globin genes during human erythroid cell maturation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. C. Mahajan and S. M. Weissman DNA-dependent adenosine triphosphatase (helicaselike transcription factor) activates beta -globin transcription in K562 cells Blood, January 1, 2002; 99(1): 348 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Francastel, W. Magis, and M. Groudine Nuclear relocation of a transactivator subunit precedes target gene activation PNAS, September 26, 2001; (2001) 211444898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Persons, E. R. Allay, D. E. Sabatino, P. Kelly, D. M. Bodine, and A. W. Nienhuis Functional requirements for phenotypic correction of murine {beta}-thalassemia: implications for human gene therapy Blood, May 15, 2001; 97(10): 3275 - 3282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Sabatino, A. P. Cline, P. G. Gallagher, L. J. Garrett, G. Stamatoyannopoulos, B. G. Forget, and D. M. Bodine Substitution of the Human beta -Spectrin Promoter for the Human Agamma -Globin Promoter Prevents Silencing of a Linked Human beta -Globin Gene in Transgenic Mice Mol. Cell. Biol., November 1, 1998; 18(11): 6634 - 6640. [Abstract] [Full Text]
|
|
|
|
|
|
A. Reik, A. Telling, G. Zitnik, D. Cimbora, E. Epner, and M. Groudine The Locus Control Region Is Necessary for Gene Expression in the Human beta -Globin Locus but Not the Maintenance of an Open Chromatin Structure in Erythroid Cells Mol. Cell. Biol., October 1, 1998; 18(10): 5992 - 6000. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. T. Lam and E. H. Bresnick Identity of the beta -Globin Locus Control Region Binding Protein HS2NF5 as the Mammalian Homolog of the Notch-regulated Transcription Factor Suppressor of Hairless J. Biol. Chem., September 11, 1998; 273(37): 24223 - 24231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Amrolia, W. Gabbard, J. M. Cunningham, and S. M. Jane Maximal Activity of an Erythroid-specific Enhancer Requires the Presence of Specific Protein Binding Sites in Linked Promoters J. Biol. Chem., May 29, 1998; 273(22): 13593 - 13598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Langdon and R. E. Kaufman Gamma-Globin Gene Promoter Elements Required for Interaction With Globin Enhancers Blood, January 1, 1998; 91(1): 309 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Bouhassira, K. Westerman, and P. Leboulch Transcriptional Behavior of LCR Enhancer Elements Integrated at the Same Chromosomal Locus by Recombinase-Mediated Cassette Exchange Blood, November 1, 1997; 90(9): 3332 - 3344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Ferry, S. B. Baliga, C. Monteiro, and B. S. Pace Globin Gene Silencing in Primary Erythroid Cultures. AN INHIBITORY ROLE FOR INTERLEUKIN-6 J. Biol. Chem., August 8, 1997; 272(32): 20030 - 20037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Marini, K. Chan, L. Casula, Y. W. Kan, A. Cao, and P. Moi hMAF, a Small Human Transcription Factor That Heterodimerizes Specifically with Nrf1 and Nrf2 J. Biol. Chem., June 27, 1997; 272(26): 16490 - 16497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Hargrove, E. F. Vanin, G. J. Kurtzman, and A. W. Nienhuis High-Level Globin Gene Expression Mediated by a Recombinant Adeno-Associated Virus Genome That Contains the 3' gamma Globin Gene Regulatory Element and Integrates as Tandem Copies in Erythroid Cells Blood, March 15, 1997; 89(6): 2167 - 2175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Elnitski, W. Miller, and R. Hardison Conserved E Boxes Function as Part of the Enhancer in Hypersensitive Site 2of the beta -Globin Locus Control Region. ROLE OF BASIC HELIX-LOOP-HELIX PROTEINS J. Biol. Chem., January 3, 1997; 272(1): 369 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Fiering, E Epner, K Robinson, Y Zhuang, A Telling, M Hu, D I Martin, T Enver, T J Ley, and M Groudine Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals that it is not essential for proper regulation of the beta-globin locus. Genes & Dev., September 15, 1995; 9(18): 2203 - 2213. [Abstract] [PDF]
|
|
|
|
|
|
P. J. Amrolia, J. M. Cunningham, P. Ney, A. W. Nienhuis, and S. M. Jane Identification of Two Novel Regulatory Elements within the 5`-Untranslated Region of the Human [IMAGE][IMAGE]-Globin Gene J. Biol. Chem., May 26, 1995; 270(21): 12892 - 12898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Elnitski, J. Li, C. T. Noguchi, W. Miller, and R. Hardison A Negative Cis-element Regulates the Level of Enhancement by Hypersensitive Site 2 of the beta -Globin Locus Control Region J. Biol. Chem., February 23, 2001; 276(9): 6289 - 6298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Francastel, W. Magis, and M. Groudine Nuclear relocation of a transactivator subunit precedes target gene activation PNAS, October 9, 2001; 98(21): 12120 - 12125. [Abstract] [Full Text] [PDF]
|
|