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Nucleic Acids Research, 1991, Vol. 19, No. 1 11-15
© 1991

GENOME STRUCTURE AND MAPPING

Locating transcribed and non-transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy

Marc Thiry and Lydia Thiry-Blaise1

1Laboratoire de Génétique appliquée, Université Libre de Bruxelles, Centre de Recherches industrielles 24 rue de I'lndustrie, B-1700-Nivelles, Belgium Laboratoire de Biologie cellulaire et tissulaire, Institut A. Swaen, Université de Liége 20 rue de Pitteurs, B-4020-Liége, Belgium

Received October 16, 1990. Revised December 4, 1990. Accepted December 4, 1990.

Immunoelectron microscopy and in situ hybridization have been used to investigate the precise location of transcribed and non-transcribed rDNA spacer sequences. Whereas a 5'-extemal transcribed spacer sequence is predominantly visualized in the flbrillar centers of nucleoli, a non-transcribed spacer sequence is preferentially detected in the interstices, in close contact with the flbrillar centers and which interrupt the surrounding dense fibrillar component. Occasionally these two spacers are also observed In clumps of dense nucleolus-associated chromatln. These observations provide insights into the organization of rlbosomal repeats within the nucleolus.


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