Skip Navigation

This Article
Right arrow Print PDF (2052K)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Li, Y.
Right arrow Articles by Sugiura, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, Y.
Right arrow Articles by Sugiura, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1991, Vol. 19, No. 11 2893-2896
© 1991

MOLECULAR BIOLOGY

Nucleic acid-binding specificities of tobacco chloroplast ribonucleoproteins

Yuqing Li and Masahiro Sugiura*

Center for Gene Research, Nagoya University Chikusa, Nagoya 464-01, Japan

*To whom correspondence should be addressed

Received March 14, 1991. Revised May 8, 1991. Accepted May 8, 1991.

Many ribonucleoproteins (RNPs) are involved in the regulation of gene expression at the posttranscriptional level. We previously isolated three nuclear-encoded RNPs from tobacco chloroplasts. Here we report their binding specificities for various nucleic acids. The three RNPs synthesized in vitro were subjected to binding assays using ssDNA, dsDNA and four ribonucleotide homopolymers. The affinities for ribonucleotide homopolymers are higher than those for ssDNA and dsDNA, suggesting that they bind preferentially to RNA in vivo. All three RNPs show high specificities for poly(G) and poly(U) in the presence of 1.2–1.8M NaCI, providing additional evidence for the similarity to HeLa hnRNP proteins. Possible involvement of these RNPs in the post-transcriptional regulation of chloroplast gene expression is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Tillich, S. L. Hardel, C. Kupsch, U. Armbruster, E. Delannoy, J. M. Gualberto, P. Lehwark, D. Leister, I. D. Small, and C. Schmitz-Linneweber
Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs
PNAS, April 7, 2009; 106(14): 6002 - 6007.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Nakamura, M. Ohta, M. Sugiura, and M. Sugita
Chloroplast Ribonucleoproteins Function as a Stabilizing Factor of Ribosome-free mRNAs in the Stroma
J. Biol. Chem., January 5, 2001; 276(1): 147 - 152.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.