Nucleic Acids Research, 1991, Vol. 19, No. 11 2955-2960
© 1991
MOLECULAR BIOLOGY |
Interaction of protein SRP19 with signal recognition particle RNA lacking individual RNA-helices
Department of Molecular Biology, The University of Texas Health Center at Tyler PO Box 2003, Tyler, TX 75710, USA
Received February 21, 1991. Revised May 8, 1991. Accepted May 8, 1991.
Derivatives of human SRP-RNA were constructed by site-directed mutagenesis and tested for their ability to interact with protein SRP19. An RNA missing helix 6 barely interacts with SRP19, while the helix 8-deletion mutant retains much binding capability. A mutant RNA consisting just of helix 6 also binds the protein, but not as well as the unaltered molecule. SRP19 interacts to a full extent with the fourth mutant RNA composed of helices 6, 7, 8 and a portion of helix 5. It is concluded that helix 6—and not helix 8—is the major SRP19 binding site. Helices 7, 8 and portions of helix 5 contribute to the formation of a functional site. These results agree with data suggesting a proximity of helix 6 and the conserved part of SRP-RNA.
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