Nucleic Acids Research, 1991, Vol. 19, No. 12 3221-3228
© 1991
MOLECULAR BIOLOGY |
Cooperation between upstream and downstream elements of the adenovirus major late promoter for maximal late phase-specific transcription
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de I'INSERM, Faculté de Médecine 11 rue Humann, 67085 Strasbourg Cedex, France
* To whom correspondence should be addressed
Received April 12, 1991. Revised May 13, 1991. Accepted May 13, 1991.
Transcription from the adenovirus major late promoter (MLP) is greatly stimulated during lytic infection, after replication of the viral DNA has started. This replicationdependent activation has previously been shown to be mediated by a positive regulatory cellular proteln(s). Binding of this factors) to sequence elements (DE1 and DE2), located between positions +76 and +124, with respect to the MLP transcriptlonal startsite, is detected only after the onset of DNA replication. Using a cellfree transcription system which mimics the late phase induction of the MLP and DNA binding assays, we now present evidence showing that maximal stimulation also depends on the MLP upstream element (UE), without Involving increased DNA binding activity of the corresponding factor (UEF) during the lytic cycle. Our results Indicate that the upstream and downstream elements act cooperatively on transcription efficiency, although no direct interactions between the cognate factors could be demonstrated. These observations strongly suggest that the elevated rate of transcription originating at the MLP startsite, late in infection, results from the simultaneous action of factors bound at the upstream and downstream elements onto a common target within the basal transcription machinery.
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