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Nucleic Acids Research, 1991, Vol. 19, No. 12 3325-3330
© 1991

MOLECULAR BIOLOGY

A new allele of the duplicated 27kD zein locus of maize generated by homologous recombination

O.Prem Das, Eduard Poliak, Kathleen Ward and Joachim Messing*

Waksman Institute, Rutgers, the State University of New Jersey Piscataway, NJ 08855-0759, USA

* To whom correspondence should be addressed

Received March 8, 1991. Revised May 6, 1991. Accepted May 6, 1991.

The 27kD zein storage protein locus in many Inbred lines of maize consists of a tandem duplication of 12kb, with an expressed gene In each repeat, termed A and B. A single-copy allele with only the A gene can be generated from this duplication in particular stocks of the maize inbred line A188 by a mitotlc process that includes a crossover at the 3' regions of the two genes (1). Here, we characterize a second single-copy allele with only the B gene, found In different stocks of A188. This allele arises from a homologous recombination at the highly conserved 5' regions of the two repeats, and cloning and sequencing of this allele define the crossover region. The A and B genes In the duplicated allele were previously shown to be expressed at different levels; this difference remains unchanged in either recombinant allele. Therefore, the crossover points of these two recombinant alleles define the borders of cis-acting sequences responsible for the differential expression of the two genes.


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