Nucleic Acids Research, 1991, Vol. 19, No. 13 3543-3547
© 1991
MOLECULAR BIOLOGY |
Identification of nuclear factor 
EF1 and its binding site essential for lens-specific activity of the 1-crystallin enhancer
Department of Molecular Biology, School of Science, Nagoya University Nagoya 464-01, Japan
* To whom correspondence should be addressed
Received April 16, 1991. Revised June 5, 1991. Accepted June 5, 1991.
The lens-specific reglatory element of the 
1-crystallin enhancer lies within the core segment (Goto et al., (1990) Mol. Cell. Biol. 10, 935–964). The element was allocated within the 55 bp long HN fragment of the core. Block-wise base substitutions were introduced to the 55 bp and their effect on the enhancer activity of the multimers in lens cells was examined. By base sequence alteration of either of the contiguous blocks 5 and 6, with their original sequence of TTGCT and C-ACCT, respectively, enhancer activity was totally lost. A lens nuclear factor EF1 was found which bound specifically to the base sequences defined by the blocks. DNA binding activity very similar to EF1 was also found in extracts of tissues other than lens, suggesting that EFI participates in lens-specific regulation through tissue-dependent modification or interaction with other factors.
+Present address Division of Biology 156-29, California Institute of Technology, Pasadena, CA 91125, USA
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